LA BORATORY IDE NTIFICATION OF DERMATOPHYTES USING PROTOPLAST HYBRIDIZATION

la boratory ide ntification of dermatophytes using protoplast hybridization

in this study techniques for laboratory identification of dermatophyte fungi through protoplast hybridization were established. firstly, auxotrophic mutants of different species of microsporum and trichophyton were induced and identified. secondly, protoplasts from these mutants were isolated by digestion of their mycelium with novozyme 234 using caci, (o.4m) as an osmotic stabilizer and glycin...

Somatic hybridization in the moss Physcomitrella patens using PEG-induced protoplast fusion.

As an alternative to sexual crossing, protoplasts from two strains of moss (Physcomitrella patens) can be hybridized using polyethylene glycol (PEG). Although the efficiency is low, it requires no sophisticated apparatus. Hybrids are readily obtained using complementary auxotrophic mutants or strains with transgenic antibiotic resistance markers. It is now routine to obtain hybrids using transg...

Dermatophytes

they contain small pieces of food, this likely contributes to their chance performance in their choices between strips in a support problem task, even in the simplest configurations. I should add that many of the problems encountered when comparing distantly related species may also apply to comparing members of the same taxonomic group or even the same species in different labs. Prosocial choi...

Intraspecific Hybridization of Populus euphratica Oliv. Using in vitro Technique

Observations and Suggestions for Improving Somatic Hybridization by Plant Protoplast Isolation, Fusion, and Culture

De (Triticum aestivum): In vitro production and utilization of doubled haploids, p. 101–124. In: Y.P.S. Bajaj (ed.). Biotechnology in agriculture and forestry. vol. 12: Haploids in crop improvement I. Springer-Verlag, Berlin. Powell, W., P.D.S. Caligari, and J.M. Dunwell. 1986. Field performance of lines derived from haploid and diploid tissues of Hordeum vulgare. Theor. Applied Genet. 72:458–4...

Genomic Linkage Analysis of Iranian Clinical Isolates of Dermatophytes Fungi Using the RAPD-PCR

Dermatophytes are a group of keratinophilic fungi capable of invading keratinized tissues (skin, hair and nails). They cause dermatophytosis (commonly known as tinea or Ring worm) in human and animals. In this report, DNA similarities and genomic linkage of 40 dermatophytes strains was obtained from different universities, were studied by random amplified polymorphic DNA (RAPD–PCR) using 11 ra...